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RNA-seq comparing LGp40 versus CDp40 pre-treated bone marrow derived macrophages after HDM challenge

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NIAID Data Ecosystem2026-05-01 收录
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To investigate the effect of GAPDH from Lactobacillus gasseri (LGp40) and Clostridium difficile (CDp40) in HDM challenged bone marrow derived macrophage (BMDM), BMDMs were pre-cultured with 10 ug/mL of LGp40 or CDp40 for 6 hours, and then re-stimulated with 100 ug/mL of HDM for another 6 hours. Afterward, harvested cells were washed twice with sterile PBS, and were then directly prepared for cDNA amplification and RNA-Seq library construction.

创建时间:
2023-03-31
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