16S and 23S ribosomal RNA gene fragments sequenced by a MinION nanopore system were used to obtain sub-species resolution of datasets from a D6322 mock bacterial microbiome. Correct strain resolution
*The restriction endonuclease (R.E.) site included in each primer is underlined and the corresponding R.E. indicated in the adjacent column. #The amplicons generated with a given set of PCR primers ar
C. kwangsiensis RNA was used to construct cDNA libraries with fragment a length of 200 bp. Then, paired-end sequencing was performed using an Illumina HiSeq TM 2500 following the manu
Table S3. Primer list. The sequence surrounding NNN-islands in the CRBO_1381 assembly scaffold was entered into Primer3 with default settings (GC clamp = 1) to find suitable primersets for PCR product
