miR-146a Suppresses SUMO1 Expression and Induces Cardiac Dysfunction in Maladaptive Hypertrophy

Table I. Calcium transient and contractility parameters of cardiomyocytes infected with Ad_pre-mir-146a or Ad_decoy-146a. Top table, isolated mouse cardiomyocytes were infected with Ad_pre-mir-146a (MOI=50) for 24h. Data represent the mean ± s.e.m. in all panels (n=100-150 cells/4 hearts). Bottom table, isolated mouse cardiomyocytes were infected with Ad_decoy-146a (MOI=50) for 24h. Data represent the mean ± s.e.m. in all panels (n=100-150 cells/4 hearts). Table II. Echocardiographic parameters of mice injected with rAAV9_pre-mir-146a. Data represent the mean ± s.e.m. of cardiac functional parameters 4 weeks post-injection. Table III. Hemodynamic parameters of mice injected with rAAV9_pre-mir-146a. Data represent the mean ± s.e.m. of cardiac functional parameters 4 weeks post-injection. Max dPdt, maximum dP/dt; Min dPdt, minimum dP/dt. Table IV. Echocardiographic parameters of mice injected with rAAV9_decoy-146a. Data represent the mean ± s.e.m. of cardiac functional parameters 4 weeks post-injection. Table V. Hemodynamic parameters of mice injected with rAAV9_decoy-146a. Data represent the mean ± s.e.m. of cardiac functional parameters 4 weeks post-injection. Table VI. Echocardiographic parameters of SUMO1 TG mice injected with rAAV9_pre-mir-146a. Data represent the mean ± s.e.m. of cardiac functional parameters 8 weeks post-injection. NL, Negative littermate; SUMO1 TG, Cardiac-specific Cre/loxP-conditional Sumo1-transgenic mice Table VII. Cardiac cellular composition. Isolated cardiac cells were sorted with FACS and analyzed. Data represent the mean ± s.e.m. (n=5).