In vivo response of EpCAM+ pancreatic cancer cells to gemcitabine plus anti-LIF neutralizing antibody treatment
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The goal of this study is to investigate the molecular mechanisms of LIF action on pancreatic cancer cells in the classical pancreatic ductal adenocarcinoma mouse model KrasLSL-G12D;Tp53f/f;Rosa26LSL-Luc;Pdx1-Cre mice EpCAM+ pancreatic cancer cells were isolated from pancreatic tumors developped in KrasLSL-G12D;Tp53f/f;Rosa26LSL-Luc;Pdx1-Cre mice treated with either gemcitabine plus control IgG or gemcitabine plus anti-LIF antibody by FACS Overall design: The KrasLSL-G12D;Tp53f/f;Rosa26LSL-Luc;Pdx1-Cre mice at 42~43 days of age were treated with either gemcitabine plus control IgG or gemcitabine plus anti-LIF antibody for five days (50mg/kg BW gemcitabine on day 1 and day4, 25 mg/kg BW control IgG or anti-LIF mAb on day 1,3,5), and whole tumors from each treated mice were individually dissected out on day 6, dissociated into single cell suspension by enzyme digestion, and EpCAM+ pancreatic cancer cells were purified by FACS
