Ct values for a skeletal muscle cDNA control demonstrate no plate-to-plate variability.

For each qPCR plate, a skeletal muscle cDNA control sample was included to assess plate-to-plate variability for every primer pair. For the Tissue Panel experiment Ct values represent three skeletal muscle cDNA biological replicates in addition to the control skeletal muscle cDNA sample. Alternatively spliced transcripts constitute approximately 30% of steady-state Actg1 mRNA in adult skeletal muscle. Two technical replicates were averaged for every biological replicate in qPCR experiments.