Transcriptome analysis of methylparaben treated H1299 lung carcinoma cells

Purpose: finding differential transcripts usage in environmentally harzardous chemical, methylparaben Methods: transcriptome analysis by the RNA-seq via mRNA pull-down Results: We found that methylparaben increases cellular toxicity in H1299 cells. Also, over thousands of retained intron events were detected from the RNA-seq analysis. Conclusions: Methylparaben increases cytotoxic effects in H1299 cells through the caspase-3 pathway and changes the context of trascript through the splicing regulation mechanisms. H1299 cells were treated with methylparaben and all experiments were duplicated.