RNA-Seq analysis of 3 month old all Ret-positive (EGFP-labeled) dorsal root ganglia L345 or L6S1 neurons

The goal of this set is to build expression data using FACS isolated Ret-positive cells harvested from a Ret-EGFP reporter mouse (3 month old). To enrich for bladder projecting neurons L6S1 DRGs were FACS isolated. L3,4, 5 lumbar levels were also FACS isolated to aid in differential expression of Ret-positive populations from L6S1 and L345 and help enrich bladder Ret-positive neurons. The series further includes these populations from males and females to uncover sex based differences. RNA was extracted from 3 month FACS isolated dorsal root ganglia dissected from Ret-EGFP C57BL/6J mice (EGFP knocked-in Ret locus-heterozyotes) and sequenced using Illumina Hiseq 2500 platform.本数据集旨在以3月龄Ret-EGFP报告基因小鼠为材料，通过荧光激活细胞分选(Fluorescence-Activated Cell Sorting, FACS)分离Ret阳性细胞，进而构建其表达谱数据。为富集膀胱投射神经元，研究人员对L6S1节段背根神经节(Dorsal Root Ganglion, DRG)开展FACS分离；同时还对L3、L4、L5腰段背根神经节进行FACS分离，以辅助对比L6S1与L345节段Ret阳性细胞群的差异表达，助力富集膀胱Ret阳性神经元。本数据集还纳入了雌雄小鼠来源的上述细胞群，以探究基于性别的表达差异。研究人员从Ret-EGFP C57BL/6J小鼠（EGFP敲入Ret位点的杂合子小鼠）中解剖获取背根神经节，经FACS分选后提取RNA，并利用Illumina HiSeq 2500平台完成测序。