RNA-SEQ analysis of E14.5 ALL Ret-positive (EGFP-labeled) FACSd dorsal root ganglia neurons.

The goal of this set is to build expression data using FACS isolated Ret-positive cells harvested from a Ret-EGFP reporter mouse (embryonic day E14.5). These can be used as a time point in comparative analysis of ontogeny of genes expressed at various developmental time points. RNA was extracted from E14.5 FACS isolated dorsal root ganglia dissected from Ret-EGFP C57BL/6J mice (EGFP knocked-in Ret locus-heterozyotes) and sequenced using Illumina Hiseq 2500 platform. Refer to protocols on the GUDMAP website under Jain.本数据集旨在利用从E14.5天胚胎Ret-EGFP报告基因小鼠（Ret-EGFP reporter mouse）中收获的、经荧光激活细胞分选术（Fluorescence-Activated Cell Sorting，FACS）分离的Ret基因阳性细胞构建基因表达谱数据。该数据集可作为不同发育时间点下基因表达个体发生比较分析的参照时间点样本。实验材料取自Ret-EGFP C57BL/6J小鼠（增强型绿色荧光蛋白（Enhanced Green Fluorescent Protein，EGFP）敲入Ret基因位点的杂合子小鼠），从其E14.5天胚胎中解剖分离背根神经节（dorsal root ganglia），经FACS分选后提取总RNA，并使用Illumina HiSeq 2500测序平台完成转录组测序。具体实验方案可参阅GUDMAP网站上Jain相关的实验流程。