BRP peptide detection in human CSF (mass spectrometry data)

Targeted metabolomics measurements were performed using an Agilent Q-TOF LC–MS instrument to verify that BRP is endogenously secreted at detectable levels in humans. We quantitated the m/z intensity of the endogenous peptide in human cerebrospinal fluid (CSF) relative to the synthesized peptide. The raw mass spectrometry (MS1 spectra) data are deposited as Agilent .d file for the synthesized peptide (BRP_amide.d) and the human CSF preparation (humanCSFprep.d). Quantification of BRP was done by manual integration (AUC) in Agilent Mass Hunter software and compared to a standard curve to estimate the CSF concentration. The data demonstrates the detection of endogenous amidated BRP in human CSF at 24 minutes at levels around 3 nM.