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An Optimized Suspension Trapping (S-Trap) Method for Phosphoproteomics Sample Preparation

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NIAID Data Ecosystem2026-05-01 收录
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The performance of the S-Trap protocol for phosphoproteomics studies for proteome and phosphoproteome was systematically evaluated in big-scale and small-scale samples. The results showed the existing protocol was problematic for phsophopeptides enrichment. After employing optimized S-Trap methodology, more peptides and phosphopeptides are found in the application for extracellular vesicles.

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2023-06-19
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