Meta-analysis of glioblastoma tissue RNA-seq datasets points to ferroptosis pathway dysregulation

Glioblastoma is a grade IV glioma of heterogeneous nature which complicates disease pathophysiology and biomarker research. Thus, the aim of our meta-analysis is to identify long noncoding RNAs (lncRNAs) and protein coding genes (PCGs) that are differentially expressed over different glioblastoma tissue datasets. Small RNA-seq of glioblastoma tissues was also performed to identify differentially expressed microRNAs (miRNAs) relative to paired controls. Fresh frozen brain tissue samples from patients with glioblastoma (n = 17) and normal controls from tumor-adjacent normal tissue (n = 3) were collected from Biobank Antwerp (University Hospital of Antwerp (UZA), Antwerp, Belgium; ID: BE 71030031000). The tissues then underwent small RNA-seq to identify differentially expressed microRNA relative to paired controls.