Determining AdVP transduction efficiency in HeLa cells

Quantification of AdVP transduction levels in HeLa cells Conclusion: Quantification of transduction levels showed that applying AdVPs at a multiplicity of infection (MOI) at or above 3000 vector genome copies per cell (GC cell-1) led to transgene expression in virtually all HeLa cells. Notes: HeLa cells were treated with the indicated amounts of AdVP.mCherry. The frequencies of transduced cells and the protein expression levels per transduced cell (left and right graph, respectively), were assessed by mCherry-directed flow cytometry at 3 days post-transduction. Mock-transduced cells were used to set fluorescence background thresholds.