RNAseq anaysis of RTF2 MEF cells

Purpose: To identify gene expression in RTF2 null cells compared to WT cells Method: Total RNA for mRNA Seq, NextSeq High Output 75 SR Results: 57 genes significantly downregulated and 7 genes significantly upregulated in RTF2 deficient MEFs Overall design: WT MEFs were compared to RTF2 null MEFs for transcriptional changes. RTF2 Null (Rtf2-/lox + Cre) MEFs are compared to WT (Rtf2+/+ + Cre) littermate controls in triplicate. Two different sets of MEFs (isolated from different mothers) were used. Cre was retrovirally induced in cell 72 hours prior to cell collection and RNA extraction.