Gene expression profiling of IR-/-, IGF-1R-/- (dKO) newborn epidermis

Analysis of newborn mouse epidermis lacking the expression of Insulin receptor (IR) and Insulin like growth factor 1 receptor (IGF-1R). Results show that IR/IGF-1R signalling control epidermal morphogenesis. RNA was isolated from newborn mouse epidermis.Gene expression profiling was on on Affymetrix 430-2.0 platform.本研究针对胰岛素受体（Insulin receptor, IR）与胰岛素样生长因子1受体（Insulin-like growth factor 1 receptor, IGF-1R）表达缺失的新生小鼠表皮组织开展分析。结果显示，IR/IGF-1R信号通路调控表皮形态发生。实验中从新生小鼠表皮组织中分离得到RNA，基因表达谱分析采用Affymetrix 430-2.0芯片平台完成。