Single cell transcriptomics of human brain epileptic lesions identifies pro-inflammatory immune mechanisms based on interlacing cellular immune networks

We analysed 85780 single cells from epileptic brain tissues using Cellular Indexing of Transcriptomes and Epitopes by Sequencing (CITE-seq) to dissect the architecture of the immuno-transcriptome of the diseased epileptic human brain. dissect the architecture of the immuno-transcriptome of the diseased epileptic human brain. Our approach uncovered in all patients, well organized pro-inflammatory microenvironment pivoting on activated, pro-inflammatory microglia in a closely-knit network where resident cells attract and manipulate in a pro-inflammatory fashion infiltrating innate and adaptive immune cells. These findings were corroborated by ligand-receptor interactome analysis, which demonstrated potential mechanisms of infiltration and by evidence of direct interactions between microglia and T cells likely contributing to pathogenesis. Immune cells Isolated from human brain tissue from epilepsy Patients analyzed ---------------------------------------------------------------- Authors state "We are not allowed to deposit Human Patients sequence data to archives."