The central clock suffices to drive the majority of circulatory metabolic rhythms

This dataset was generated to investigate the independence of the central clock in regulating hepatic transcriptional rhythms. The core clock gene Bmal1 was knocked out using a floxed STOP codon (KO mice). By crossing these mice with Syt10-Cre mice we were able to rescue the Bmal1 expression in the suprachiasmatic nucleus (RE mice). These mice were compared with mice expressing Bmal1 in all tissues (WT). Livers were collected at 6 time points over the circadian 24-hour period.本数据集旨在探究中枢时钟对肝脏转录节律的调控独立性。研究人员利用携带flox终止密码子（floxed STOP codon）的基因编辑策略，构建了Bmal1敲除小鼠（KO小鼠）。将该品系小鼠与Syt10-Cre小鼠杂交后，可在下丘脑视交叉上核（suprachiasmatic nucleus）中恢复Bmal1的表达，由此获得RE小鼠（rescue小鼠）。将这些RE小鼠与全身组织均表达Bmal1的野生型小鼠（WT小鼠）进行对照比较。最终在昼夜节律24小时周期内的6个时间点采集各组小鼠的肝脏组织。