ChIP-seq analysis of RNA polymerase I inhibitor effect on Pol I and Pol II binding

Ribosome biosynthesis is essential for cancer growth and proliferation. This dependency is therapeutically exploitable by blocking the rate-limiting step, RNA polymerase I (Pol I) transcription. Here we investigated the effects of RNA Pol I inhibitor on binding of Pol I RPA1 and Pol II Rpb1 on genomic DNA. Overall design: We treated LNCaP prostate cancer cells with either DMSO vehicle or 1 microM BMH-21 for 6 hrs.