*Letters F and R after the primer name indicate forward and reverse orientation, respectively. †Theoretical melting temperature (Tm) calculated using the MWG Oligo Property Scan (MOPS).
We examined the global gene expression profile of PLCγ1/PLCγ2 double-deficient CLPsand B cell progenitors by RNA-seq analysis. Overall design: CLPs (Lin-IL-7R+) and B cell progenitors (B220+IgM-IL-7R+
Table S3. Primer list. The sequence surrounding NNN-islands in the CRBO_1381 assembly scaffold was entered into Primer3 with default settings (GC clamp = 1) to find suitable primersets for PCR product