(high-temp) No 2. Data Preparation (16S rRNA/ITS) Output

Output files from the No 2. Data Preparation Workflow page of the SWELTR high-temp study.Workflow objects:data_prep_ssu18_wf.rdata: contains all variables and phyloseq objects from the 16s rRNA part of data prep pipeline. To see the Objects, in R run load("data_prep_ssu18_wf.rdata", verbose=TRUE)data_prep_its18_wf.rdata: contains all variables and phyloseq objects from the ITS part of data prep pipeline. To see the Objects, in R run load("data_prep_its18_wf.rdata", verbose=TRUE)Additional files:For convenience, we also include raw phyloseq objects(before removing contaminants, etc.), as well as individual ASV, taxonomy, sample data tables. Each zip file contains the following:*_ps.rds: raw phyloseq object.*_full_seq_table.txt: Tab-delimited text file of ASV table.*_full_tax_table.txt: Tab-delimited text file of taxonomy table.*_sam_tab.txt: Tab-delimited text file of sample table.*_full.fasta: Fasta formatted file of all ASV sequences*_ps_work_o.rds: Phyloseq object after removing contaminants. data_prep_wf_ssu.zip: data packet for 16S rRNA data.data_prep_wf_its.zip: data packet for ITS data.Source code for the workflow can be found here:https://github.com/sweltr/high-temp/blob/main/data-prep.Rmd