Chromosome-Scale Genome Sequence of Alternaria alternata Causing Tobacco Brown Spot

<em>Alternaria alternata </em>is an economically important pathogen that can cause severe plant dis? eases in various crops, such as citrus, pear, strawberry, and tobacco. Here, we report on the genome sequence of <em>A. alternata </em>DZ causing tobacco brown spot disease in most tobacco? producing areas. A total of 11 contigs, including 10 essential chromosomes and 1 mitochon? dria genome, were assembled by combining Oxford Nanopore and Illumina sequencing tech? nologies. All 10 assembled chromosomes enabled detection of at least one of the telomeric terminal repeat sequences CCCTAA/TTAGGG. The assembled chromosomes were a total of 34.11 Mb in length with a GC content of 50.95%, whereas the mitochondrial genome was 50,595 bp in length with a GC content of 29.16%. We were unable to identify any evidence of conditionally dispensable chromosomes in the genome of <em>A. alternata </em>DZ, nor were we able to locate any ACT gene orthologs. This genome resource will provide valuable insights for comparative genomics research of the <em>Alternaria </em>genus and serve as a useful resource in investigations of plant-pathogen molecular interactions.