RNAseq of cultured neonatal rat ventricular cardiomyocytes treated with siRNA for Btg1 and Btg2 knockdown.

Total RNA was isolated from n=3 culture wells per siRNA treatment group, from 6 independent primary neonatal rat cardiomyocyte isolations. RNA was pooled to obtain n=3 independent samples per siRNA treatment group for sequencing. The siRNA treatment groups were= siScr (Scramble control), siBtg12 (Btg1/Btg2 double knockdown), siBtg1 (Btg1 single knockdown), siBtg2 (Btg2 single knockdown).本研究从6次独立的原代新生大鼠心肌细胞分离实验中，针对每个siRNA（小干扰RNA）处理组收取3个培养孔的细胞以提取总RNA；随后将同组的RNA混合，得到每个siRNA处理组共3份独立的测序样本。本次实验设置的siRNA处理组包括：siScr（Scramble阴性对照）、siBtg12（Btg1与Btg2双基因敲低组）、siBtg1（Btg1单基因敲低组）以及siBtg2（Btg2单基因敲低组）。