Mycobacterium tuberculosis strain:MNPK Genome sequencing and assembly. Mycobacterium tuberculosis strain:MNPK

Complete genome sequence of MTB resourced from Pakistani patients following genome wide analysis via cloud computing to understand the genetic makeup and to investigate the evolutionary lineages, virulent genes and horizontal gene transfer to investigate the disease epidemiology. Transcriptome sequence of MTB grown under in vitro (Löwenstein–Jensen medium) and in vivo (artificial sputum medium host mimic medium) to characterize the gene expression profiling under host specific and host independent conditions. This genotyping data of the mycobacterium associated with diseases and drug resistance will be extremely beneficial in defining the accurate treatment strategies and identification of MTB. The study will go a long way in bringing excellent repute globally into the medical research of Pakistan.