Single cell RNA sequence data from intrahepatic immune cells of CHB(chronic hepatitis B) mice

We successfully established a novel immunocompetent mouse model of CHB(chronic hepatitis B) at the immune-active phase by a simple combination of a Fumarylacetoacetate hydrolase (FAH) knockout mouse, hydrodynamic tail vein injection, and sleeping-beauty transposon system. The employment of single-cell RNA sequence in this model revealed the comprehensive intrahepatic immune landscape in the immune-active phase, which contributes to the persistence of HBV infection. Our model also made it possible to profile the intrahepatic immune response to the immunomodulators. Hepatic immune cells were analysed by scRNA-seq at the chronic inflammation phase .

本研究成功构建了一种新型免疫健全型免疫活性期慢性乙型肝炎（chronic hepatitis B, CHB）小鼠模型，构建策略为联合应用延胡索酰乙酰乙酸水解酶（Fumarylacetoacetate hydrolase, FAH）敲除小鼠、流体动力学尾静脉注射与睡美人转座子系统（sleeping-beauty transposon system）。通过在该模型中开展单细胞RNA测序（single-cell RNA sequence），我们揭示了免疫活性期肝内的全面免疫图谱，该图谱与HBV（hepatitis B virus, HBV）感染的持续存在密切相关。本模型同时实现了肝内针对免疫调节剂的免疫应答特征解析。此外，本研究在慢性炎症阶段通过scRNA-seq对肝内免疫细胞进行了分析。