Unraveling the Role of Srsf1 in Brown Adipose Tissue Thermogenic Function through Single-Nucleus RNA Sequencing.
收藏Alliance of Genome Resources2026-08-01 收录
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Single-nucleus RNA sequencing reveals that Srsf1 absence alters the cell type composition and differentiation trajectories of BAT. Interscapular brown adipose tissue was harvested from 8-week-old control and adipose tissue-specific Srsf1-knockout mice for experiment. In order to obtain single-cell suspension, fresh tissue samples were cut and lysed in nucleus lysis solution. The nuclei obtained were subsequently used for sequencing.
