Methane oxidation coupling to heavy metal and microplastic transformations for biochar-mediated landfill cover soil

Microbial analysisFor microbial composition, 16S rDNA sequence analysis was conducted, involving DNA extraction, PCR amplification, high-throughput sequencing using the Illumina MiSeq platform, and subsequent sequencing data processing following the procedures reported by Huang et al. (2019). Additionally, Metagenomic sequencing, genome assembly, Gene prediction, taxonomy, and functional (KEGG) annotation were analyzed on the online platform of Majorbio Cloud Platform (www.majorbio.com).