RNAseq of wheat

All samples were frozen in liquid nitrogen immediately after sampling. Two biological replicates of 23 samples were used to transcriptome profiling. Generally, each sample was ground in liquid nitrogen and total RNA was extracted with RNAprep Pure Plant Plus Kit (TIANGEN BIOTECH (BEIJING) CO., LTD). Sequencing libraries were generated using MGIEasy Fast RNA Library Prep Set for DNBSEQTM sequencers. The library preparations were sequenced on an MGISEQ-2000 platform and 150 bp paired-end reads were generated. Cutadapt was used to remove the adapter of the original short-read sequence and obtain the original short-read sequence of 10-10.5G for each sample, and Salmon was utilized for calculating the raw expression quantification of each gene.