*Letters F and R after the primer name indicate forward and reverse orientation, respectively. †Theoretical melting temperature (Tm) calculated using the MWG Oligo Property Scan (MOPS).

NIAID Data Ecosystem60

Data from: Blocking human contaminant DNA during PCR allows amplification of rare mammal species from sedimentary ancient DNA

DNA分析

PCR技术

Analyses of degraded DNA are typically hampered by contamination, especially when employing universal primers such as commonly used in environmental DNA studies. In addition to false-positive results,

DataONE2011-09-09 更新60

Additional file 10: of Expanding the biodiversity of Oenococcus oeni through comparative genomics of apple cider and kombucha strains

基因组学

PCR技术

Table S3. Primer list. The sequence surrounding NNN-islands in the CRBO_1381 assembly scaffold was entered into Primer3 with default settings (GC clamp = 1) to find suitable primersets for PCR product

Mendeley Data2024-06-27 更新70

Primers used and PCR amplicons generated in this study.

分子生物学

PCR技术

*The restriction endonuclease (R.E.) site included in each primer is underlined and the corresponding R.E. indicated in the adjacent column. #The amplicons generated with a given set of PCR primers ar

NIAID Data Ecosystem60