"Mapping information-rich genotype-phenotype landscapes with genome-scale Perturb-seq" Replogle et al. 2022 processed Perturb-seq datasets

This dataset includes data from three Perturb-seq experiments described in Replogle et al. 2022 (https://doi.org/10.1016/j.cell.2022.05.013): K562 genome-scale perturb-seq sampled at day 8 post-transduction (K562_gwps) K562 essential-scale perturb-seq sampled at day 6 post-transduction (K562_essential) RPE1 essential-scale perturb-seq sampled at day 7 post-transduction (rpe1) For each dataset, there are four processed Perturb-seq files in AnnData format (_{https://anndata.readthedocs.io/en/latest/}). Raw, single-cell expression data for genes expressed at >0.01 UMI per cell (named $pop_raw_singlecell_01.h5ad) Raw, pseudo-bulk expression data for genes expressed at >0.01 UMI per cell (named $pop_raw_bulk_01.h5ad) gemgroup Z-normalized single-cell expression data for genes expressed at >0.01 UMI per cell (named $pop_normalized_singlecell_01.h5ad) gemgroup Z-normalized pseudo-bulk expression data for genes expressed at >0.01 UMI per cell (named $pop_normalized_bulk_01.h5ad) In the anndata format, the .var annotation details genes while the .obs annotation details single-cells/pseudobulk populations.本数据集包含来自Replogle等人2022年发表的三项扰动测序（Perturb-seq）实验的数据（https://doi.org/10.1016/j.cell.2022.05.013），具体包括：转导后第8天取样的K562全基因组尺度扰动测序（K562_gwps）、转导后第6天取样的K562必需基因尺度扰动测序（K562_essential），以及转导后第7天取样的RPE1必需基因尺度扰动测序（rpe1）。针对每个数据集，均包含四种经处理的扰动测序文件，格式为AnnData（_{https://anndata.readthedocs.io/en/latest/}）：单基因每细胞UMI计数大于0.01的原始单细胞表达数据，文件名为$pop_raw_singlecell_01.h5ad；单基因每细胞UMI计数大于0.01的原始伪批量表达数据，文件名为$pop_raw_bulk_01.h5ad；gemgroup Z标准化的、单基因每细胞UMI计数大于0.01的单细胞表达数据，文件名为$pop_normalized_singlecell_01.h5ad；gemgroup Z标准化的、单基因每细胞UMI计数大于0.01的伪批量表达数据，文件名为$pop_normalized_bulk_01.h5ad。在AnnData格式中，.var注释用于标注基因信息，.obs注释用于标注单细胞/伪批量群体信息。