The Signature of Glucocorticoid Receptor -Dependent Hypertrophic Transcriptome in Cardiomyocytes [RNA-Seq]

We examined Glucocorticoid receptor binding sites in isolated neonatal cardiomyocytes treated with Dexamethasone (100nM) for 1hr or Ethanol using GR-ChIP-Seq. In addition to determine the change in the resulting transcriptional status of genes we performed RNAseq in cardiomyocytes treated with Dexamethasone (100nM) or Ethanol for 1hr or 24hrs.本研究采用GR-ChIP-Seq技术，对经100nM地塞米松处理1小时或经乙醇处理的离体新生心肌细胞中的糖皮质激素受体（Glucocorticoid receptor, GR）结合位点进行了检测。此外，为探究经上述处理后基因转录状态的变化，我们还对分别经100nM地塞米松或乙醇处理1小时、24小时的心肌细胞开展了RNA测序（RNAseq）分析。