BFG-PCA and BFG-Y2H are methods utilizing synthetic DNA barcodes for detection of binary protein-protein interactions. Here we screened >11,000 bait/prey pairs to compare the interactions detected by
Additional file 2: Table S1. NEK10 protein interactors identified in HEK293T cells treated or not with zeocin by IP-MS/MS. Proteins marked with an asterisk: *: Retrieved from the databank by IIS platf
Comparison of BioID (24h labeling) and ultraID (10 min labeling) for PDB-MS (POI = Ago2, control protein = Rab11). 4 biological replicates per condition.
In this project, we designed a SUMO-specific bimolecular fluorescence complementation screen framework, namely biFC-SUMO, to systematically identify the SUMO interaction proteins and SUMO-modified pro