RNA-seq analysis of gene expression during and after exposure to hydroxyurea in WT and mec1 cells in Saccharomyces cerevisiae

We previously demonstrated that inactivation of the replication checkpoint via a mec1 mutation led to chromosome breakage at replication forks initiated from virtually all origins of replication, after transient exposure to hydroxyurea (HU), an inhibitor of ribonucleotide reductase我们此前已证实，在短暂接触羟基脲（HU）——一种核糖核苷酸还原酶（ribonucleotide reductase）抑制剂——后，通过mec1突变使复制检验点（replication checkpoint）失活，会导致几乎所有复制起始位点（origins of replication）所启动的复制叉（replication forks）处发生染色体断裂。