Meiosis timecourse Rap1-depletion Rap1 ChIP-chip

To confirm that Rap1-depletion resulted in decreased binding of Rap1 to its target sites, we performed compared Rap1 ChIP-chip experiments in respiratory cells (pre-meiotic/YPA 20 hours) depleted (plus doxycycline for 12 hours) or not depleted (minus doxycycline) of Rap1为验证Rap1敲低会导致Rap1与其靶位点的结合能力下降，我们在呼吸细胞（减数分裂前/YPA培养20小时）中开展了对比性Rap1染色质免疫沉淀芯片（ChIP-chip）实验，分别设置Rap1敲低组（加入强力霉素处理12小时）与非敲低对照组（不加入强力霉素）。