RNA-Seq of DRG and dorsal cord after nerve injury

Genes are up and down regualted in DRG and spinal dorsal cord after peripheral nerve injury Overall design: WT male adult with sciatic and femoral nerve transection 7 days, RNA was purified from ipilateral or contralateral L4-L6 DRGs or lumbar spinal dorsal cords外周神经损伤后，背根神经节（Dorsal Root Ganglion, DRG）与脊髓背角内的基因呈现上调与下调表达。总体设计：对成年雄性野生型（Wild Type, WT）小鼠施行坐骨神经与股神经切断术，术后7天，分别从术侧及对侧的L4-L6节段背根神经节或腰段脊髓背角中提取纯化RNA。

搜集汇总

背景与挑战

背景概述

该数据集包含4个小鼠RNA-Seq实验，旨在研究神经损伤后背根神经节和脊髓背角的基因表达变化。所有实验均使用Illumina HiSeq 2000平台进行单端测序，覆盖损伤侧和对侧样本，数据以fastq格式公开提供，适用于转录组分析。