RNA sequencing of T cells in the genetically encoded mouse model of T cell anergy (W131AOTII)

We found that gene signatures characteristic of unresponsive cells were strongly enriched in peripheral W131AOTII CD4 T cells but not in CD4 single positive (CD4SP) thymocytes. Furthermore, loss of Cbl-b in W131AOTII mice significantly altered gene expression profile associated with improved T cell responsiveness. Overall design: RNA sequencing of T cells isolated from OTII, Cbl-b-/-OTII, W131AOTII and Cbl-b-/-W131AOTII to investigate the possible downstream signaling pathways that cbl-b controls and rescues T cell responsiveness in T cell anergy.