In the study presented here POSH2 was overexpressed in the MCF10AT breast cancer cells. RNA sequencing profiling of mRNA levels was performed in the control and overexpression cells.
The mean length of input reads was 49 bp and two mismatches were allowed by GSNAP. Reads hitting more than one locus in the genome were excluded. Each exon-exon junction was supported by at least one
A549 and MDA-MB-231 cells were transfected with the Bloom-specific siRNA. Total RNA was extracted using Trizol at 48 hours after transfection. RNA-SEQ was carried out to profile the gene expression in
