Effect of LPS-MCM and Hemin-MCM on gene expression of astrocytes

To investigate the transcriptional changes occurred in astrocytes after inflammatory or hemorrhagic stroke in vitro, we performed RNA sequencing (RNA-seq) of resting-MCM treated astrocytes, LPS-MCM treated astrocytes, and Hemin-MCM treated astrocytes. Overall design: Astrocytes were isolated from P0 C57BL/6 mice and were treated with conditioned medium from LPS-stimulated microglial (LPS-MCM) or hemin (the degradation product of hemoglobin)-stimulated microglial (Hemin-MCM). Comparative gene expression profiling analysis of RNA-seq data for resting-MCM treated astrocytes, LPS-MCM treated astrocytes, and Hemin-MCM treated astrocytes, each group has 3 separate duplications.