Deciphering the Developmental Dynamics of the Mouse Liver Transcriptome

This study uses Whole Transcriptome Shotgun Sequencing (RNA-Seq) data to interrogate the developmental dynamics of the C57BL/6 mouse liver transcriptome. We analyze gene expression patterns in the developing mouse liver over 12 distinct time points from late embryonic stage (2 days before birth) to maturity (60 days after birth). Biological functions deemed significant at different developmental stages are identified and their leverage is mapped over time. Upstream regulators modulating these gene expression patterns are determined and their regulatory networks are described along with putative transcriptional modules associated with gene regulation. Several genes that were alternatively spliced over time were identified. Previously uncharacterized isoforms were found for several genes. We analyze gene expression patterns in the developing mouse liver over 12 distinct time points from late embryonic stage (2 days before birth) to maturity (60 days after birth). Three replicates per time point.本研究采用全转录组鸟枪法测序（Whole Transcriptome Shotgun Sequencing，RNA-Seq）数据，解析C57BL/6小鼠肝脏转录组的发育动态特征。我们对从胚胎晚期（出生前2天）至成年（出生后60天）的12个不同时间点的发育中小鼠肝脏的基因表达模式展开分析。鉴定出不同发育阶段的显著生物学功能，并绘制其随时间推移的调控权重图谱。确定调控上述基因表达模式的上游调控因子，阐述其调控网络及与基因调控相关的推定转录模块。本研究同时鉴定出多个随时间发生可变剪接的基因，并发现了多个此前未被注释的基因异构体。我们再次对从胚胎晚期（出生前2天）至成年（出生后60天）的12个不同时间点的发育中小鼠肝脏的基因表达模式进行分析，每个时间点设置三次生物学重复。