Mouse tissue atlas

We used state of the art mass spectrometry (MS) and RNA sequencing (RNA-Seq) to provide the first integrated proteomic, phosphoproteomic and transcriptomic atlas of the animal model Mus musculu . We measured 66 murine pancreatic ductal adenocarcinoma cell lines (66 proteomes and 66 phosphoproteome) and 41 healthy tissues (41 proteomes, 41 phosphoproteome, and 29 transcriptomes). The employed MS-based and bioinformatics strategy identified >17,000 proteins and >50,000 phosphorylation sites, providing expression evidence for ~76% of the 22,437 protein-coding genes reported in UniProtKB. The RNA-Seq strategy resulted in the quantification of 21,261 unique gene that were expressed in at least one of the 29 sequenced tissue.本研究采用国际领先的质谱法（mass spectrometry, MS）与RNA测序（RNA sequencing, RNA-Seq）技术，首次构建了模式生物小家鼠（Mus musculus）的整合蛋白质组、磷酸化蛋白质组及转录组图谱。我们共检测了66株小鼠胰腺导管腺癌细胞系（对应66份蛋白质组与66份磷酸化蛋白质组样本）以及41份健康组织样本（对应41份蛋白质组、41份磷酸化蛋白质组与29份转录组样本）。所采用的基于质谱的生物信息学分析策略，共鉴定得到超过17000种蛋白质与超过50000个磷酸化位点，为UniProt知识库（UniProtKB）中收录的22437个蛋白质编码基因中约76%的基因提供了表达证据。通过RNA测序策略，我们对29份测序组织中至少在1份组织内表达的21261个独特基因完成了定量分析。