File S1 - A Molecular Evolution Approach to Study the Roles of Tropomyosin in Fission Yeast

Contains: Figure S1. Sequence alignment of the fungal tropomyosin genes used in our study. Figure S2. Marker reconstitution mutagenesis strategy. Table S1. List of species and accession numbers. Table S2. ω values of the S. pombe cdc8 sequence (161 residues). Table S3. Analysis of cdc8-27 overexpressing wildtype and mutant Cdc8p at 35°C. Table S4. List of primers used for MRM and pET vector cloning. Table S5. Strains used in this study. References. (DOCX)