(A-D) Knock-in efficiencies (% of total cell population) as determined by flow cytometry analysis for Fig 1C, 1D, 1F and 1G. (E) Fluorescent signals reported in Fig 4C. Values are corrected as explain
Cells nucleofected with either CRISPR/Cas9 alone or with CRISPR/Cas9 and a homologous donor template were subjected to targeted high-throughput sequencing of the beta-globin locus. Here, one sample wi
a: Counted only the founders where progeny was analyzed by both methods. b: Calculated assuming only one embryo in the pools with mutant peaks is heterozygous.
