Single-cell transcriptional response of placenta to the ablation of Caveolin-1 in mice: insights into the regulation of brain-placental axis [scRNA-Seq]

This study investigated gene expression of placental cells in a mouse model lacking Caveolin 1 (Cav1). Single-nuclei RNA-seq was performed with day-15 placenta of WT and Cav1-null mice. Day-15 placenta of WT (C56BL6/J) and Cav1-null (B6.Cg-Cav1tm1Mls/J) mice was used for snRNA-seq analysis by 10x Genomics.本研究针对缺失小窝蛋白1（Caveolin 1，Cav1）的小鼠模型，探究了胎盘细胞的基因表达特征。研究采用野生型（Wild Type，WT）与Cav1基因敲除（Cav1-null）小鼠的妊娠第15天胎盘组织，开展单细胞核RNA测序（Single-nuclei RNA-seq，snRNA-seq）分析。其中，野生型小鼠品系为C56BL6/J，Cav1基因敲除小鼠品系为B6.Cg-Cav1tm1Mls/J，相关测序分析由10x Genomics平台完成。