Total number of similar amino acid sites between the different groups of sarcopterygians which have been affected by positive-destabilizing during cladogenesis.

Abbreviations: A- Amphibians, L- Lungfishes, C- Coelacanths; (pK') - Equilibrium constant (ionization of COOH), (Ra) - Solvent accessible reduction ratio, (an) - Power to be at the N-terminal, (Hp) - Surrounding hydrophobicity, (H) - Hydropathy, (Ko) - Compressibility, (F) - Mean r.m.s. fluctuation displacement, (Ht) - Thermodynamic transfer hydrophobicity, (P) – Polarity. The table shows clearly that the total number of amino acid sites commonly affected by positive selection is much higher between amphibians and lungfishes (A-L) when compared to the other groupings. Hence we come to a conclusion that amphibians and lungfishes are very closely related. Also pK' and Ra have affected the genes to a maximum extent unlike other properties. Please note that only the major amino acid properties are listed in the table (Refer to Table S2 for all the details).