Transcription profiling of proximal and distal muscle groups in dysferlin-deficient mice (C57BL/10.SJL_Dysf) to understand selective pattern of muscle involvement in muscular dystrophies

By comparing the transcriptome from proximal (quadriceps femoris, QF) and distal (tibialis anterior, TA)muscle groups in dysferlin deficient mouse muscle (the SJL mutation bred onto C57BL/10 to produces C57BL/10.SJL_Dysf) with proximal and distal muscle groups from control C57BL/10 mice of an equivalent age (3-weeks old, prior to the onset of overt pathology) we aim to address the issues of muscle selectivity in this this form of muscular dystrophy.本研究将肌集钙蛋白（dysferlin）缺陷型小鼠（将SJL突变回交至C57BL/10背景，构建得到C57BL/10.SJL_Dysf小鼠）的近端（股四头肌，QF）、远端（胫骨前肌，TA）肌肉组织的转录组（transcriptome），与同年龄段（3周龄，显性病理发作前）的对照C57BL/10小鼠的对应近端、远端肌肉组织的转录组进行比较分析，旨在阐明该型肌营养不良症的肌肉选择性致病机制。