Mechanomics AFM Validation - TGBC CAV1 KD esiRNA

Entry is a part of Mechanomics collection (10.6084/m9.figshare.c.5399826) that contains materials supporting findings presented in the following manuscript: Urbanska, M., Ge, Y. et al., De novo identification of universal cell mechanics gene signatures BiorXiv: https://doi.org/10.1101/2021.04.26.441418; first published: 27th April 2021, last updated: 4th Nov 2024 eLife: https://doi.org/10.7554/eLife.87930; first published: 23rd March 2023, last updated: 27th Dec 2024 This dataset contains mechanical measurement of CAV1 knock-down in TGBC18TKB (TGBC) adenocarcinoma cell line performed using esiRNA from Eupheria Biotech GmbH. Measurements were performed using atomic force microscopy (AFM) with a followings setup: Nanowizard 4 (JPK Instruments, Germany). Tip-less cantilevers (PNP-TR-TL, nominal spring constant k = 0.08 N m−1, Nanoworld, Switzerland) decorated with a polystyrene bead of 5-µm diameter (PS-R-5.0, microParticles, Germany) each were used as the indenters. Following sub-folders are included under each sample: • Calibration - contains data acquired during cantilever calibration • Ind - indentation curves in .jpk-force format • Ind_processed-yyyy.mm.dd-hh.mm.ss - results of processing of indentation curves • Osc - records of oscillatory measurements in .jpk-force format • Osc_processed-yyyy.mm.dd-hh.mm.ss - results of processing of oscillatory measurements • Pics - bright-field images of cells acquired during the measurements, cantilever is located above the cell of interest