Cell proliferation of P. brasiliensis stimulated by low concentrations of H2O2 or nitrite is suppressed in the presence of FPT III inhibitor.

(A) Pb18 yeast cells were seeded in a 6-well culture plate subjected to a 24-h period of starvation with F12 medium and pretreated with 250 µM FPT III and stimulated with 0.1 mM H2O2 or 0.25 µM nitrite at pH 5.6 for 5 h at 37°C. Cells were plated in BHI medium at 37°C for 10 days (n = 6 at each point) and CFU were counted. (B) Same as in (A), but cells (1.5×105) were cultured in the YPD medium (n = 4 each point) and after 4 days the fungal growth was determined by counting in a Neubauer chamber. The graphs show the mean CFU or number of cells ± SD for each sample. The results represent three independent experiments. Statistically significant samples are indicated (p