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Decreased sperm production in aged testes and functional sperm defects in aged epididymides lead to decreased fertility in male mice.

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To analyze mRNA expressions in aged mouse testes and cauda epididymides at genome wide level, we performed mRNA-Seq on these bulk samples at 2, 18, and 24 months. Bulk testes and cauda epididymides in wild-type C57BL/6J mice at 2, 18, and 24 months (five biological replicates for each organ) were placed in TRIzol, homogenized, and obtained the total RNAs.

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