No safe renal warm ischemia time-The molecular network characteristics and pathological features of mild to severe ischemia reperfusion kidney injury. No safe renal warm ischemia time-The molecular network characteristics and pathological features of mild to severe ischemia reperfusion kidney injury

Purpose: we performed comparative RNA-seq analyses to identify molecular network characteristics of kidney injury at different ischemia times Methods: Unilateral clamping of the left renal pedicle plus contralateral nephrectomy was performed. Briefly, mice were anesthetized, right kidney was excised. The left kidney was clamped for indicated minutes (0min for sham-24h group, 16min for uIRIx16min-24h group, 18minfor uIRIx18min-24h group, 20min for uIRIx20min-24h group, 22min for uIRIx22min-24h group, 24min for uIRIx24min-24h group, 26min for uIRIx26min-24h group, 28min for uIRIx28min-24h group, 30min for uIRIx30min-24h group). Mice were placed on 37°C heating platform while operation, ischemia and before awakening. At 24h after ischemia, mice were anesthetized and blood was taken to detect creatinine, kidney was harvest for PAS staining and mRNA seq. Results: After sequence, the clean reads rate of all samples were ≥98%.The quality of the assembled transcriptome is good enough for functional annotation and further analysis. Conclusions: We performed RNA-sequencing (RNA-Seq) analyses to identify molecular network characteristics of kidney injury at different ischemia time. Overall design: We performed RNA-sequencing (RNA-Seq) on kidney of 9 groups. Grant No. : 82100713 Grant title:The role and mechanism of PFKFB3 in promoting fibrosis after acute kidney injury by regulating the metabolic reprogramming of renal tubular epithelial cells. Name of the funding source: the National Natural Science Foundation of China.

研究目的：本研究通过比较RNA测序（RNA-seq）分析，旨在明确不同缺血时长下肾损伤的分子网络特征。
研究方法：采用左侧肾蒂单侧夹闭联合对侧肾切除术构建肾损伤模型。具体操作如下：对小鼠实施全身麻醉后摘除右侧肾脏，随后对左侧肾脏夹闭指定时长（假手术24h组夹闭0min，uIRIx16min-24h组夹闭16min，uIRIx18min-24h组夹闭18min，uIRIx20min-24h组夹闭20min，uIRIx22min-24h组夹闭22min，uIRIx24min-24h组夹闭24min，uIRIx26min-24h组夹闭26min，uIRIx28min-24h组夹闭28min，uIRIx30min-24h组夹闭30min）。手术操作、缺血过程及小鼠苏醒前，将其置于37℃加热平台维持体温稳定。缺血24h后，再次麻醉小鼠并采集血液以检测肌酐水平，同时摘取肾脏组织用于过碘酸雪夫（PAS）染色及mRNA测序。
研究结果：测序结果显示，所有样本的clean reads率均≥98%；组装得到的转录组质量优异，可满足功能注释及后续分析需求。
研究结论：本研究通过RNA测序（RNA-seq）分析，明确了不同缺血时长下肾损伤的分子网络特征。
整体实验设计：本研究对9组小鼠肾脏组织开展RNA测序（RNA-seq）分析。
资助信息：项目批准号：82100713；项目名称：PFKFB3通过调控肾小管上皮细胞代谢重编程促进急性肾损伤后纤维化的作用及机制；资助单位：国家自然科学基金（National Natural Science Foundation of China）。

搜集汇总

背景与挑战

背景概述

该数据集是一个关于小鼠肾脏缺血再灌注损伤的转录组学研究，通过RNA-seq分析不同缺血时间（0-30分钟）下的分子网络特征和病理变化。数据集包含27个实验样本，数据量约为32 Gbases，所有样本的clean reads率≥98%，转录组质量良好，适用于功能注释和深入分析。研究由国家自然科学基金资助，旨在探索肾脏损伤的分子机制。