IVT-SAPAS: low-input and rapid method for sequencing alternative polyadenylation sites. Homo sapiens

Here, based on our method of SAPAS (sequencing alternative polyadenylation sites), we developed a new method called IVT-SAPAS by integrating in vitro transcription and magnetic bead-based purification and size selection. With the new method, as low as 200 ng of total RNA is sufficient to obtain 3' end library, benefiting from the good linear amplification of in vitro-transcription and high recovery rate of magnetic bead-based method.