This study aimed to understand the abundance and diversity of ammonia/methane oxidizing bacteria communities using DPeTTs-PCR method. amoA/pmoA genes were collected by 454-pyrosequencing from multiple
A custom mcrA gene (Methyl-coenzyme M reductase A) reference database with 39,544 sequences and assigned taxonomy for studying methanogens with metabarcoding. Sequences in database were extracted from
In this study, a simulation was carried out using a lab modified anaerobic jar converted digester with that of a commercial/pilot scale anaerobic digester. Taxonomic profiling of biogas producing comm
Amplicon libraries were prepared as described in Han et al. 2020 (https://doi.org/10.1111/1462-2920.15115) and involved the same mcrA primer combination as for qPCR. Sequencing was done on an Illumina
