Detection of membranous and soluble forms of gp130 and gp80 and detection of IL-6 in tumour cell lines.

Membrane staining of gp130 and gp80 and intracellular staining of IL-6 were performed on 2×105 cells as described in materials and methods with 1 µg/mL primary antibody. Results were expressed as the mean % of positive cells of two independent experiments realized in duplicate. IL-6, soluble gp130 (sgp130) and sgp80 were measured by ELISA on 100 µL supernatants as described in materials and methods. Results were expressed as the mean±SD of three independent experiments realized in duplicate (pg/mL).